Dental pulp stem cells (DPSCs) maintain nice promise for utilization in tissue restore and regenerative drugs.
Routinely, culture media used for culturing stem cells are supplemented with animal serum for promoting progress and profitable upkeep of stem cells.
However, there may be a rising demand for optimizing a well-defined culture media that might safely improve the efficacy and reproducibility of the cultured cells. In this research, we aimed toward optimizing a serum-free/xeno-free culture medium.
A cocktail of numerous dietary supplements supposed to counterpoint DPSCs proliferation in outlined concentrations was designed.
It consisted of recombinant human fundamental fibroblast progress issue (hbFGF), insulin transferrin selenium (ITS), ascorbic acid (vitamin C), Beta mercaptoethanol and ldl cholesterol.
The impact of this optimized media on the proliferation of DPSCs was assessed by MTT assay and circulate cytometric evaluation (FACS) of early apoptotic marker annexin V. Expression of stemness-related genes (OCT4, SOX and NANOG) was assessed by qRT-PCR.Proliferation outcomes by MTT illustrated a important improve in the proliferation fee of DPSCs cultured in the proposed media.
FACS evaluation of annexin V expression was nil. Expression of OCT4, SOX and NANOG genes was additionally up-regulated.
The proposed mixture of dietary supplements utilized in the proposed culture media efficiently elevated the proliferation potential of DPSCs along with enhancing the stemness properties.
Thus, it may be thought of a promising and secure substitute to conventional animal derived dietary supplements like fetal bovine serum (FBS).
[Retracted] Serum‑free‑medium‑kind mesenchymal stem cell culture supernatant exerts a protecting impact on A549 lung epithelial cells in acute lung damage induced by H2O2.
The authors want to retract their analysis article entitled ‘Serum‑free‑medium‑kind mesenchymal stem cell culture supernatant exerts a protecting impact on A549 lung epithelial cells in acute lung damage induced by H2O2’, revealed in Oncology Reports 40, 3033‑3039, 2018.
After the publication of this text, the authors have develop into involved that there have been flaws of their research design which have known as into query the reported outcomes.
On repeating sure of the experiments, the authors discovered that the Nrf2‑Keap1‑ARE signaling pathway solely has a function in the lung epithelial cell damage mannequin, whereas it doesn’t serve a function in the A549 mannequin.
Further research are required to validate the function of the Nrf2‑Keap1‑ARE signaling pathway and the apoptosis‑related proteins. In explicit, the outcomes introduced in Fig. 5, displaying the distinction between Bax and Bcl‑2, look like incorrect. For these causes, the authors have determined to retract the article from the publication.
All the named authors on the paper comply with this retraction. The authors sincerely apologize for any inconvenience that may consequence from the retraction of this text. [the original article was published in the Oncology Reports 40: 3033‑3039, 2018; DOI: 10.3892/or.2018.6656].