Construction of a quencher-free cascade amplification system for highly specific and sensitive detection of serum circulating miRNAs.

Circulating miRNAs are a newly emerging class of noninvasive biomarkers, and the accurate quantification of their expression is essential to the biological research and early clinic diagnosis.

Herein, we demonstrate the construction of a quencher-free cascade amplification system for highly sensitive detection of serum circulating miRNAs. The target miRNA can hybridize with the linear probe to induce the cyclic strand displacement amplification (SDA) (cycle I) for the production of the binding probes.

The binding probe can subsequently react with the 2-aminopurine (2-AP)-hairpin probe to induce the recycling exonuclease cleavage of 2-AP-hairpin probes (cycle II), releasing the triggers and 2-AP molecules simultaneously. The released trigger can hybridize with the free linear probe to start new cycles I and II amplifications. Through multiple rounds of cascade amplifications, a large number of 2-AP molecules are released, generating an enhanced fluorescence signal.

Human kaliuretic peptide,KP ELISA Kit

201-12-1324 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human kaliuretic peptide(KP)ELISA Kit

GA-E1340HM-48T 48T
EUR 289

Human kaliuretic peptide(KP)ELISA Kit

GA-E1340HM-96T 96T
EUR 466

Human kaliuretic peptide(KP)ELISA Kit

QY-E02370 96T
EUR 361

This method exhibits a large dynamic range of 8 orders of mag-nitude and a detection limit of 0.16 aM. It can differentiate a single-base mismatch in miR-486-5p, quantify miR-486-5p in lung cancer cells at various stages, and even discriminate the expressions of serum circulating miR-486-5p in healthy persons from that in nonsmall-cell lung carcinoma (NSCLC) patients.

Moreover, this assay can be rapidly carried out in one step under isothermal condi-tion in a label-free manner, holding promising applications in point-of-care diagnosis and prognosis of lung cancers.